Radioimmunoassay (RIA)
This technique which is very sensitive and is used to measure the concentrations of antigens. Radioimmunoassay technique is a specific and sensitive test, but this technique need a special equipment, which is costly. Substances used in this test are radioactive, so special precautions are to be observed.
Radioimmunoassay or RIA is one of the tests used to help the clinician determine the levels of hormones in a patient. Estrogen is one of these hormone. The level of estrogen is important to be measured because an increase or decrease of its level is unhealthy and may be detrimental to the patient.
Principle:
In this test, antigens which are of known amount or quantity are made radioactive. These antigens, which are now radioactive, are then mixed with antibodies of that antigens. Which result the two to bind chemically to one another. Then, a serum sample from a patient which contain an unknown amount or quantity of the same antigens is then added. This cause the unlabeled antigens from the patient's serum to compete with the radiolabeled antigens for binding sites in the antibody.
Reference:
Wikipedia-RIA
This technique which is very sensitive and is used to measure the concentrations of antigens. Radioimmunoassay technique is a specific and sensitive test, but this technique need a special equipment, which is costly. Substances used in this test are radioactive, so special precautions are to be observed.
Radioimmunoassay or RIA is one of the tests used to help the clinician determine the levels of hormones in a patient. Estrogen is one of these hormone. The level of estrogen is important to be measured because an increase or decrease of its level is unhealthy and may be detrimental to the patient.
Principle:
In this test, antigens which are of known amount or quantity are made radioactive. These antigens, which are now radioactive, are then mixed with antibodies of that antigens. Which result the two to bind chemically to one another. Then, a serum sample from a patient which contain an unknown amount or quantity of the same antigens is then added. This cause the unlabeled antigens from the patient's serum to compete with the radiolabeled antigens for binding sites in the antibody.
Reference:
Wikipedia-RIA
