A SHORT QUIZ ON RADIOIMMUNOASSAY (RIA)

1. This technique measures the concentration of which substance?

2-4. In RIA, what are the three components that should be present for the test to be performed?

5. The concentration of the labeled antigen is (directly/ inversely) proportional to the natural antigen.

6-7. Who developed the RIA technique?

8. One of the developers of RIA received a Nobel Prize for what hormone?

9. What is the reagent or material used for labeling antigens?

10. More of the unlabeled antigens are binding to the antibodies when the unlabeled antigen's concentration is (increased/ decreased).



CASE STUDY

A 20 year old female noticed that there is a lump found on her throat. She also observed that she gained weight in the past weeks. Her hands felt cold and that she was always nervous. She went to the doctor and had her blood tested. Radioimmunoassay was the test performed. The results showed that she has hyperthyroidism.

• What is/are the hormones tested that confirmed the patient's condition?
• What are the expected results in the said condition?
• Are there other test/s that should be done for the confirmation of the patient's condition? Give a brief explanation.
  
• What other signs & symptoms seen in patients with hyperthyroidism?
• In your own words, state the principle of radioimmunoassay.

Reference:
WIKI-RIA